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マウス白血病細胞への薬剤耐性遺伝子の移入と分化誘導
https://meilib.repo.nii.ac.jp/records/348
https://meilib.repo.nii.ac.jp/records/348d313440f-522c-47cb-851c-feed387f9228
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
mgjc.journal.1990.11hidaka,yamagoe.. (1.4 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-02-19 | |||||
| タイトル | ||||||
| タイトル | マウス白血病細胞への薬剤耐性遺伝子の移入と分化誘導 | |||||
| タイトル | ||||||
| タイトル | DRUG-RESISTANCE GENE TRANSFER INTO CULTURED-MURINE ERYTHRO-LEUKEMIA CELLS : PREFERENTIAL ACTIVITY IN INDUCING DIFFERENTIATION OF THE TRANSDUCTED CELLS IN VITRO BY DIMETHYL SULFOXIDE AND VITAMIN D_3 | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
日高, 英幸
× 日高, 英幸× 山越, 智× 大石, 道夫 |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 715 | |||||
| 姓名 | HIDAKA, Hideyuki | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 716 | |||||
| 姓名 | YAMAGOE, Satoshi | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 717 | |||||
| 姓名 | OISHI, Michio | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The gene transfer into, various target cells is an important technique in genetic engineering, for such as biomass production or gene therapy because of the critical role it plays in new protein synthesis and intermediary metabolism. We demonstrate here, using a laser microinjector, the introduction of a drug-resistance gene into cultured-murine cells (TSA8). The cells were transformed to Geneticin (G418) resistance by introduction of the neomycin-resistanc gene. The transformed-murine erythroleukemia cells could be in- duced to differentiate by dimethyl sulfoxide (DMSO). Erythroid differrentiation by vitamin D_3 derivatives was examined in the cells. After the cells were cultured for 5-days with 1.0% DMSO, as much as 60% of .the cells became benzydine-positive. This differentiation was markedly inhibited by addition of the active form of vitamin D_3, 1α, 25-dihydroxyvitamin D_3 (1α, 25 (OH)_2D_3). The active vitamin D_3 was the most potent in inhibition for the DMSO-induced erythroid differentiation. These results are the same results that the vitamin D_3 derivatives and DMSO are involved in erythroid differentiation of normal murine cells, as published elsewhere. These results suggest that the transduction of neomycin-resistance genes is totally ineffective in cell growth and erythroid differentiation for murine cells. These experiments also provide a posibility.or model for future various gene replacement therapy in which functional genes can be introduced into various target cells using the laser microinjector. | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The gene transfer into, various target cells is an important technique in genetic engineering, for such as biomass production or gene therapy because of the critical role it plays in new protein synthesis and intermediary metabolism. We demonstrate here, using a laser microinjector, the introduction of a drug-resistance gene into cultured-murine cells (TSA8). The cells were transformed to Geneticin (G418) resistance by introduction of the neomycin-resistanc gene. The transformed-murine erythroleukemia cells could be in- duced to differentiate by dimethyl sulfoxide (DMSO). Erythroid differrentiation by vitamin D_3 derivatives was examined in the cells. After the cells were cultured for 5-days with 1.0% DMSO, as much as 60% of .the cells became benzydine-positive. This differentiation was markedly inhibited by addition of the active form of vitamin D_3, 1α, 25-dihydroxyvitamin D_3 (1α, 25 (OH)_2D_3). The active vitamin D_3 was the most potent in inhibition for the DMSO-induced erythroid differentiation. These results are the same results that the vitamin D_3 derivatives and DMSO are involved in erythroid differentiation of normal murine cells, as published elsewhere. These results suggest that the transduction of neomycin-resistance genes is totally ineffective in cell growth and erythroid differentiation for murine cells. These experiments also provide a posibility.or model for future various gene replacement therapy in which functional genes can be introduced into various target cells using the laser microinjector. | |||||
| 書誌情報 |
宮崎女子短期大学紀要 en : Bulletin of Miyazaki Women's Junior College 巻 17, p. 207-220, 発行日 1991-03 |
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| 出版者 | ||||||
| 出版者 | 宮崎女子短期大学 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 02898748 | |||||
